《植物生理学报》 2010, 46(3): 245-248

黄瓜中CBF1 基因的克隆及其表达分析

李丹, 蒋欣梅*, 于锡宏*

东北农业大学园艺学院蔬菜生理与设施园艺研究室, 哈尔滨 150030

通信作者：蒋欣梅；E-mail: yxh100@sohu.com, jxm0917@163.com；Tel: 451-55190300

摘　要：

以黄瓜中 RNA 为模板, RT-PCR 扩增出 CBF1 基因 cDNA 序列的部分片段。测序表明此基因与黄瓜 CBF1 基因的同 源性达 99.44%。RT-PCR 方法检测结果显示, CBF1 基因在低温和盐胁迫下的黄瓜中表达, 而在干旱和 ABA 胁迫下则不表达。

关键词：黄瓜; CBF1; RT-PCR; 基因表达

收稿：2009-11-16   修定：2010-02-10

资助：黑龙江省教育厅项目(11531019)、哈尔滨市科技创新人才研究专项基金项目(2007RFLXN004)、东北农业大学创新团队课题(CXT002-2-3)、黑龙江省教育厅寒地蔬菜生物学重点实验室。

Cloning and Expression Analysis of CBF1 from Cucumber (Cucumis sativus L.)

LI Dan, JIANG Xin-Mei*, YU Xi-Hong*

Vegetable Physiology and Installation Horticulture Laboratory, College of Horticulture, Northeast Agricultural University, Harbin 150030, China

Corresponding author: JIANG Xin-Mei; E-mail: yxh100@sohu.com, jxm0917@163.com; Tel: 451-55190300

Abstract:

The CBF1 gene were obtained by RT-PCR from cucumber (Cucumis sativus). The sequence showed high homology (99.44%) to the published sequences of CBF1. The expression patterns of the CBF1 gene in response to low temperature, drought, ABA and NaCl stresses at different time points were further investigated by using RT-PCR. The results showed that CBF1 gene could express under low temperature and NaCl treatment in cucumber. However, it could not express under drought and ABA treatment.

Key words: cucumber (Cucumis sativus); CBF1; RT-PCR; gene expression